Marchal C, Perrin D, Hofnung M
C R Seances Acad Sci D. 1979 Jan 15;288(2):275-7.
We describe a method which should allow construction of bacterial strains able to export a given protein through the cytoplasmic membrane. The principle is to fuse the structural gene of the protein to the proximal part of gene lamB, the structural gene of the lambda receptor, an outer membrane protein of E. coli K-12.
我们描述了一种方法,该方法应能构建出能够通过细胞质膜输出特定蛋白质的细菌菌株。其原理是将蛋白质的结构基因与基因lamB的近端部分融合,lamB是λ受体的结构基因,λ受体是大肠杆菌K-12的一种外膜蛋白。