供体年龄对培养的人角膜内皮细胞形态学变化的影响。
Effect of donor age on morphologic variation of cultured human corneal endothelial cells.
作者信息
Miyata K, Drake J, Osakabe Y, Hosokawa Y, Hwang D, Soya K, Oshika T, Amano S
机构信息
Miyata Eye Hospital, Miyakonojo, Japan.
出版信息
Cornea. 2001 Jan;20(1):59-63. doi: 10.1097/00003226-200101000-00012.
PURPOSE
To examine the effect of donor age on the morphologic variation of cultured human corneal endothelial cells (HCEC).
METHODS
HCEC were obtained from the remaining corneoscleral rims of seven human corneas used for penetrating keratoplasty. The donor age ranged from 2 to 75 years. Primary cultures were established from explants of the endothelial cell layer, including the Descemet's membrane, and were propagated on culture dishes coated with bovine corneal endothelial extracellular matrix. At the fourth passage, frequency distribution of cell area in the confluent monolayer was calculated and the effect of donor age on cell area was analyzed.
RESULTS
The percentage of HCEC with cell area over 2000 microm2 significantly increased with donor age (r = 0.935, p = 0.0007).
CONCLUSION
Cultured HCEC established from older donor tissue display greater heterogeneity. The use of HCEC from younger donors may be preferable to maximize the benefits of HCEC transplantation.
目的
研究供体年龄对培养的人角膜内皮细胞（HCEC）形态变化的影响。
方法
从7例用于穿透性角膜移植术的人角膜剩余角膜缘组织中获取HCEC。供体年龄范围为2至75岁。以内皮细胞层（包括Descemet膜）的外植体建立原代培养物，并在涂有牛角膜内皮细胞外基质的培养皿上进行传代培养。在第4代时，计算汇合单层中细胞面积的频率分布，并分析供体年龄对细胞面积的影响。
结果
细胞面积超过2000平方微米的HCEC百分比随供体年龄显著增加（r = 0.935，p = 0.0007）。
结论
从老年供体组织建立的培养HCEC表现出更大的异质性。使用年轻供体的HCEC可能更有利于最大化HCEC移植的益处。
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