Ishii H, Ishibashi M, Takayama M, Nishida T, Yoshida M
Respiratory Medicine, Internal Medicine, Fukuoka University, Japan.
Respirology. 2000 Dec;5(4):325-31.
Remote lung injury is induced by ischaemia/reperfusion (I/R) of the gastrointestinal tract and the liver following hypovolaemic shock. In the present study, the role of cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin (IL)-8 family, in neutrophil-mediated remote lung injury following intestinal I/R was investigated in anaesthetized rats.
The I/R group was subjected to 60 min of occlusion of the superior mesenteric artery with laparotomy, followed by 240 min of intestinal reperfusion. The sham-operated (sham) group was subjected to the same procedures with the exception of intestinal I/R.
In the I/R group, the permeability index of the lung, the neutrophil count in pulmonary vascular lavage fluid and bronchoalveolar lavage fluid (BALF), lung myeloperoxidase activity and neutrophil oxidative production were all significantly greater than those in the sham group. Cytokine-induced neutrophil chemoattractant-1 levels in blood and BALF were significantly increased at 240 min after intestinal reperfusion. There was a significant relationship between neutrophils in BALF and CINC-1 level in BALE CONCLUSION: These findings suggest that intestinal reperfusion was associated with activation and accumulation of neutrophils in the lung and resulted in remote lung injury with increased microvascular permeability. Thus, CINC-1 in BALF may induce neutrophil migration from the pulmonary vessels to the interstitium and alveolar spaces in remote lung injury after intestinal I/R.
低血容量性休克后胃肠道和肝脏的缺血/再灌注（I/R）可导致远隔性肺损伤。在本研究中，在麻醉大鼠中研究了细胞因子诱导的中性粒细胞趋化因子（CINC，白细胞介素（IL）-8家族成员）在肠道I/R后中性粒细胞介导的远隔性肺损伤中的作用。
I/R组通过剖腹术对肠系膜上动脉进行60分钟的阻断，随后进行240分钟的肠道再灌注。假手术（sham）组除不进行肠道I/R外，接受相同的操作。
在I/R组中，肺的通透性指数、肺血管灌洗液和支气管肺泡灌洗液（BALF）中的中性粒细胞计数、肺髓过氧化物酶活性和中性粒细胞氧化产物均显著高于假手术组。肠道再灌注后240分钟时，血液和BALF中细胞因子诱导的中性粒细胞趋化因子-1水平显著升高。BALF中的中性粒细胞与BALE中的CINC-1水平之间存在显著相关性。结论：这些发现表明肠道再灌注与肺中中性粒细胞的激活和积聚有关，并导致微血管通透性增加的远隔性肺损伤。因此，BALF中的CINC-1可能在肠道I/R后的远隔性肺损伤中诱导中性粒细胞从肺血管迁移至间质和肺泡腔。
