Lewin A, Bert B, Dalsgaard A, Appel B, Høi L
Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
J Basic Microbiol. 2000;40(5-6):377-84.
Vibrio vulnificus serovar E (biotype 2) strains are virulent for eels and have also been reported to cause illness in humans. Studies on the plasmid content of serovar E strains revealed the existence of a plasmid of approximately 70 kbp present in most of these strains. In this study we characterized the 70 kbp plasmids of seven biotype 2 strains isolated from seawater, diseased eels and wound infections in humans. We determined the exact size of the high molecular weight plasmids to be 68 kbp. A comparison of the plasmids of the seven strains by restriction length polymorphism and hybridization analysis showed them to be almost identical.
创伤弧菌血清型E(生物型2)菌株对鳗鱼具有致病性,也有报道称可导致人类患病。对血清型E菌株的质粒含量研究发现,大多数此类菌株中存在一个约70 kbp的质粒。在本研究中,我们对从海水、患病鳗鱼及人类伤口感染中分离出的7株生物型2菌株的70 kbp质粒进行了特性分析。我们确定这些高分子量质粒的确切大小为68 kbp。通过限制性片段长度多态性和杂交分析对这7株菌株的质粒进行比较,结果显示它们几乎完全相同。