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在对鳗鱼具有致病性的创伤弧菌菌株中发现的一种高度同源的68千碱基对质粒。

A highly homologous 68 kbp plasmid found in Vibrio vulnificus strains virulent for eels.

作者信息

Lewin A, Bert B, Dalsgaard A, Appel B, Høi L

机构信息

Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.

出版信息

J Basic Microbiol. 2000;40(5-6):377-84.

Abstract

Vibrio vulnificus serovar E (biotype 2) strains are virulent for eels and have also been reported to cause illness in humans. Studies on the plasmid content of serovar E strains revealed the existence of a plasmid of approximately 70 kbp present in most of these strains. In this study we characterized the 70 kbp plasmids of seven biotype 2 strains isolated from seawater, diseased eels and wound infections in humans. We determined the exact size of the high molecular weight plasmids to be 68 kbp. A comparison of the plasmids of the seven strains by restriction length polymorphism and hybridization analysis showed them to be almost identical.

摘要

创伤弧菌血清型E(生物型2)菌株对鳗鱼具有致病性,也有报道称可导致人类患病。对血清型E菌株的质粒含量研究发现,大多数此类菌株中存在一个约70 kbp的质粒。在本研究中,我们对从海水、患病鳗鱼及人类伤口感染中分离出的7株生物型2菌株的70 kbp质粒进行了特性分析。我们确定这些高分子量质粒的确切大小为68 kbp。通过限制性片段长度多态性和杂交分析对这7株菌株的质粒进行比较,结果显示它们几乎完全相同。

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