Hurelbrink C B, Armstrong R J, Barker R A, Dunnett S B, Rosser A E
Cambridge Centre for Brain Repair, University of Cambridge, UK.
Cell Transplant. 2000 Nov-Dec;9(6):743-9. doi: 10.1177/096368970000900601.
The use of fresh human fetal tissue in neural transplantation presents considerable logistical difficulties and limits the clinical applicability of this promising therapy. This study compared the survival of human fetal striatal tissue that had been stored for 24 h in a defined hibernation medium with that of fresh human fetal striatal tissue following xenotransplantation in a rat model of Huntington's disease (HD). Six to 7 weeks postgrafting, there was no significant difference between fresh and hibernated grafts in volume or in various striatal phenotypic markers, although there was a trend towards decreased graft volume. We conclude that short-term hibernation of this tissue is without significant adverse effects on the survival of grafted human fetal striatal tissue. This has important implications for the practical implementation of clinical neural transplant programs in HD.
在神经移植中使用新鲜的人类胎儿组织存在相当大的后勤困难，并限制了这种有前景的治疗方法的临床适用性。本研究比较了在亨廷顿舞蹈病（HD）大鼠模型中异种移植后，在特定的冬眠培养基中储存24小时的人类胎儿纹状体组织与新鲜人类胎儿纹状体组织的存活率。移植后6至7周，新鲜移植组织和冬眠移植组织在体积或各种纹状体表型标志物方面没有显著差异，尽管移植组织体积有减小的趋势。我们得出结论，这种组织的短期冬眠对移植的人类胎儿纹状体组织的存活没有显著不利影响。这对HD临床神经移植项目的实际实施具有重要意义。
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