Corchero J, Pimprale S, Kimura S, Gonzalez F J
Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Pharmacogenetics. 2001 Feb;11(1):1-6. doi: 10.1097/00008571-200102000-00001.
The sequence and organization of the CYP1A cluster on human chromosome 15 was determined. A human genomic clone from a BAC library, containing both CYP1A1 and CYP1A2 genes, was isolated and sequenced. The results of Southern blot analysis using human genomic DNA were compatible with the structure of the BAC clone. The CYP1A1 and CYP1A2 genes are separated by a 23 kb segment that contains no other open reading frames. The CYP1A1 and CYP1A2 genes are in opposite orientation, revealing that the 5' flanking region is in common between the two genes. Analysis of the sequence obtained revealed the presence of xenobiotic response elements (XREs) previously reported for CYP1A1 and CYP1A2 and several additional consensus sequences for putative XREs. The presence of all the XREs upstream of both genes suggest that some of the regulatory elements known to control CYP1A1 gene expression, could also control CYP1A2 gene expression.
确定了人类15号染色体上CYP1A基因簇的序列和组织。从一个包含CYP1A1和CYP1A2基因的BAC文库中分离出一个人类基因组克隆并进行测序。用人基因组DNA进行Southern印迹分析的结果与BAC克隆的结构相符。CYP1A1和CYP1A2基因被一个23 kb的片段隔开，该片段不包含其他开放阅读框。CYP1A1和CYP1A2基因呈相反方向，表明两个基因之间有共同的5'侧翼区域。对所得序列的分析揭示了先前报道的CYP1A1和CYP1A2的外源性反应元件（XREs）以及几个推定XREs的额外共有序列。两个基因上游所有XREs的存在表明，一些已知控制CYP1A1基因表达的调控元件也可能控制CYP1A2基因的表达。
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