利用重组蛋白片段完全重建网格蛋白篮状结构的形成：衔接蛋白对网格蛋白自组装的调控

Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly.

作者信息

Greene B, Liu S H, Wilde A, Brodsky F M

机构信息

G.W. Hooper Foundation, Department of Microbiology and Immunology, and Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, P.O. Box 0552, University of California, San Francisco, CA 94143, USA.

出版信息

Traffic. 2000 Jan;1(1):69-75. doi: 10.1034/j.1600-0854.2000.010110.x.

DOI:10.1034/j.1600-0854.2000.010110.x

PMID:11208061

Abstract

Clathrin polymerization into a polyhedral basket, surrounding budding membrane vesicles, mediates protein sorting during endocytosis and organelle biogenesis. Adaptor proteins target clathrin assembly to specific membrane sites and sequester receptors into the clathrin coat. We have reconstituted complete clathrin basket formation from recombinantly expressed fragments of clathrin and adaptors. This reconstitution reveals a hierarchy of clathrin self-assembly interactions and demonstrates that adaptors control basket formation by alignment of the distal domains of the clathrin triskelion leg through their binding to the terminal domain.

摘要

网格蛋白聚合成多面体篮筐，围绕正在出芽的膜泡，在内吞作用和细胞器生物发生过程中介导蛋白质分选。衔接蛋白将网格蛋白组装靶向特定的膜位点，并将受体隔离到网格蛋白包被中。我们利用重组表达的网格蛋白片段和衔接蛋白片段重构了完整的网格蛋白篮筐形成过程。这种重构揭示了网格蛋白自组装相互作用的层次结构，并表明衔接蛋白通过与网格蛋白三脚蛋白腿的末端结构域结合，使三脚蛋白腿的远端结构域排列来控制篮筐的形成。