Xi Q Y, Li N, Tang Y X, Meng Q Y, Yuan L, Wu C X
College of Animal Science, Jiangxi Agricultural University, Nanchang 330045, China.
Yi Chuan Xue Bao. 2001;28(1):7-14.
Molecular genetic polymorphisms were studied for MHC-B-LII beta(beta 1 exon) locus in ten breeds of chicken by PCR-RFLPs. Thirty-seven genotypes were detected in these chicken. In the 305 bp fragment derived from MHC-B-LII beta(beta 1 exon) locus digested with Hha I, EcoRV, HaeIII, Xba I, there were significant difference with the frequencies of genotype and combined genotype among individuals or breeds. Statistical results showed Hardy-Weinberg equilibrium in polymorphic sites were inconsistent among various breeds. Sequencing results suggested there are more polymorphism at the level of amino acid than at that of nucleotide. Furthermore, as antigen binding site, the highly polymorphisms of beta 1 exon sequence was a reflection to the antigen diversity of immune systems. Moreover, it was implied that there was highly association between genetic polymorphisms of beta 1 exon and reproduction performance in chicken.
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLPs）技术，对十个鸡品种的MHC-B-LIIβ（β1外显子）基因座的分子遗传多态性进行了研究。在这些鸡中检测到了37种基因型。在用Hha I、EcoRV、HaeIII、Xba I消化后，来自MHC-B-LIIβ（β1外显子）基因座的305bp片段中，个体或品种间基因型和组合基因型的频率存在显著差异。统计结果表明，不同品种多态性位点的哈迪-温伯格平衡不一致。测序结果表明，氨基酸水平的多态性比核苷酸水平的多态性更多。此外，作为抗原结合位点β1外显子序列的高度多态性反映了免疫系统的抗原多样性。此外，这意味着β1外显子的遗传多态性与鸡的繁殖性能之间存在高度关联。
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