Bolgiano B, Fowler S, Turner K, Sesardic D, Xing D K, Crane D T, Corbel M J
Bacteriology Division, National Institute for Biological Standards and Control, Potters Bar, Herts., UK.
Dev Biol (Basel). 2000;103:51-9.
A combination of spectroscopic and chromatographic methods has been used to monitor the quality and integrity of diphtheria, pertussis and tetanus toxoids (DTxd, PTxd and TTxd) which have been prepared from the toxins by formaldehyde treatment. Different processes for detoxifying all three toxins have yielded toxoids varying in their molecular size, including oligomers (associated monomers) and aggregates (high molecular weight complexes of non-specifically associated monomers). Changes in the intrinsic fluorescence spectra of the polypeptides have been observed in some sized fractions of DTxd and PTxd. Some physico-chemical changes have been observed to correlate with a loss of antigenicity. Spectroscopic and chromatographic methods are useful not only in monitoring the stability and consistency of vaccine starting materials, but can also be used to dissect heterogeneous toxoid preparations.
已使用光谱法和色谱法相结合的方法来监测通过甲醛处理从毒素制备的白喉、百日咳和破伤风类毒素（DTxd、PTxd和TTxd）的质量和完整性。对所有三种毒素进行解毒的不同工艺产生了分子大小各异的类毒素，包括寡聚体（缔合单体）和聚集体（非特异性缔合单体的高分子量复合物）。在DTxd和PTxd的一些大小分级组分中观察到了多肽固有荧光光谱的变化。已观察到一些物理化学变化与抗原性丧失相关。光谱法和色谱法不仅有助于监测疫苗起始材料的稳定性和一致性，还可用于剖析异质性类毒素制剂。
