Liu C Y, Liu J Z, Li Z Y, Liu K J
Department of Physiology, Shandong Medical University, Jinan 250012.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2000 Nov;16(4):347-9.
To investigate the effect of vagus nerve on coordinating the motion of gallbladder (GB) and sphincter of Oddi (SO) in the interdigestive period in rabbits.
Fasted for 15 h-18 h, but allowed to drink water, the rabbib were anesthetized with urethane (1.0 mg/kg, i.v.). In order to measure GB pressure, a frog bladder filled with normal saline was put into GB and connected to a transducer (TP-200T). Myoelectric signals of SO was recorded by a pair of copper electrodes.
In the interdigestive period, phasic contractions of GB (PCGB) and clusters of spike potentials of SO (CSPSO) was 1:1 correlated (Y = 0.962X + 0.587, r = 0.982, P < 0.01). That is, every PCGB was accompanied by one CSPSO. Microinjection of thyrotropin-releasing hormone (TRH, 0.8 nmol, 1 microliter) or monosodium glutamate (MSG, 2 mumol, 1 microliter) into dorsal vagal complex (DVC) enhanced the motility of GB and SO, and the 1:1 temporal relation between PCGB and CSPSO still existed. Vagotomy or intravenous injection of atropine inhibited the PCGB and the spike potentials of SO, and the 1:1 correlation between PCGB and CSPSO disappeared. The spike bursts of SO did not respond to the artificial rise of gallbladder pressure.
In the interdigestive period in rabbits, gallbladder and SO contract and relax rhythmically and simultaneously. This eoordinated motion between GB and SO is controlled by DVC via vagus nerve and peripheral M receptors.
研究迷走神经对家兔消化间期胆囊（GB）和Oddi括约肌（SO）运动协调的影响。
家兔禁食15 - 18小时，但可饮水，用乌拉坦（1.0毫克/千克，静脉注射）麻醉。为测量胆囊压力，将充满生理盐水的蛙膀胱放入胆囊并连接到传感器（TP - 200T）。用一对铜电极记录SO的肌电信号。
在消化间期，胆囊的时相性收缩（PCGB）与SO的成簇峰电位（CSPSO）呈1:1相关（Y = 0.962X + 0.587，r = 0.982，P < 0.01）。即每一次PCGB都伴有一次CSPSO。向迷走神经背核复合体（DVC）微量注射促甲状腺激素释放激素（TRH，0.8纳摩尔，1微升）或谷氨酸钠（MSG，2微摩尔，1微升）可增强GB和SO的运动，且PCGB与CSPSO之间的1:1时间关系仍然存在。切断迷走神经或静脉注射阿托品可抑制PCGB和SO的峰电位，PCGB与CSPSO之间的1:1相关性消失。SO的峰爆发放对胆囊压力的人工升高无反应。
在家兔消化间期，胆囊和SO有节律地同步收缩和舒张。GB和SO之间的这种协调运动由DVC通过迷走神经和外周M受体控制。
