孕早期自然流产孕妇循环血中的胎儿DNA。

Fetal DNA in maternal circulation of first-trimester spontaneous abortions.

作者信息

Skinner J, Luettich K, Ring M, O'Leary J J, Turner M J

机构信息

The Coombe Women's Hospital and Trinity College, Dublin, Ireland.

出版信息

Obstet Gynecol. 2001 Mar;97(3):460-3. doi: 10.1016/s0029-7844(00)01173-x.

DOI:10.1016/s0029-7844(00)01173-x

PMID:11239657

Abstract

OBJECTIVE

To establish whether fetal DNA can be identified in the maternal circulation in first-trimester spontaneous abortions.

METHODS

Women with confirmed spontaneous abortions and no histories of previous pregnancy were recruited. Peripheral venous blood samples were obtained and DNA extracted. Real-time quantitative polymerase chain reaction was done using SRY and beta-actin systems for calculating fetal and total DNA, respectively.

RESULTS

Of 25 women, SRY-specific signals were detected in 11 indicating that the abortions were male. The remaining 14 were negative for the SRY gene. Women with positive results were of similar gestational age to those who were negative (mean 68.4 and 69.0 days). Fetal:total DNA ratio was calculated for positive samples and ranged from 15.8 to 360.1 x 10(+3). Mean ratio was 99.4 x 10(+3) and median was 67.5 x 10(+3).

CONCLUSION

Fetal DNA is present in the maternal circulation of first-trimester spontaneous abortions.

摘要

目的

确定在孕早期自然流产的孕妇血液循环中是否能鉴定出胎儿DNA。

方法

招募确诊为自然流产且无既往妊娠史的女性。采集外周静脉血样本并提取DNA。分别使用SRY和β-肌动蛋白系统进行实时定量聚合酶链反应，以计算胎儿DNA和总DNA。

结果

25名女性中，11名检测到SRY特异性信号，表明流产胎儿为男性。其余14名SRY基因检测为阴性。结果为阳性的女性与阴性女性的孕周相似（平均分别为68.4天和69.0天）。对阳性样本计算胎儿DNA与总DNA的比率，范围为15.8至360.1×10(+3)。平均比率为99.4×10(+3)，中位数为67.5×10(+3)。