Kvaratskhelia M, Wardleworth B N, White M F
Centre for Biomolecular Science, St Andrews University, Fife KY16 9ST, North Haugh, UK.
FEBS Lett. 2001 Mar 2;491(3):243-6. doi: 10.1016/s0014-5793(01)02200-1.
Holliday junction resolving enzymes are required by all life forms that catalyse homologous recombination, including all cellular organisms and many bacterial and eukaryotic viruses. Here we report the identification of three distinct Holliday junction resolving enzyme activities present in two highly divergent archaeal species. Both Sulfolobus and Pyrococcus share the Hjc activity, and in addition possess unique secondary activities (Hje and Hjr). We propose by analogy with the two other domains of life that the latter enzymes are viral in origin, suggesting the widespread existence of archaeal viruses that rely on homologous recombination as part of their life cycle.
所有催化同源重组的生命形式都需要霍利迪连接点解离酶，包括所有细胞生物以及许多细菌和真核病毒。在此，我们报告在两种高度不同的古菌物种中鉴定出三种不同的霍利迪连接点解离酶活性。硫化叶菌属和嗜热栖热菌都具有Hjc活性，此外还拥有独特的二级活性（Hje和Hjr）。我们通过与生命的其他两个域进行类比推测，后一种酶起源于病毒，这表明广泛存在着依赖同源重组作为其生命周期一部分的古菌病毒。
