在福尔马林固定组织中禽肺病毒的免疫组织化学检测

Immunohistochemical detection of avian pneumovirus in formalin-fixed tissues.

作者信息

Jirjis F E, Noll S L, Halvorson D A, Nagaraja K V, Shaw D P

机构信息

Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108, USA.

出版信息

J Vet Diagn Invest. 2001 Jan;13(1):13-6. doi: 10.1177/104063870101300103.

DOI:10.1177/104063870101300103

PMID:11243355

Abstract

An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults experimentally inoculated with APV and from older turkeys infected during naturally occurring outbreaks of avian pneumovirus. Tissue was fixed in 10% buffered neutral formalin, embedded in paraffin, sectioned and stained. Inflammatory changes were observed microscopically in the mucosa and submucosa of the nasal turbinates and infraorbital sinuses of both experimentally inoculated poults and naturally infected birds. Viral antigen was detected by IHC in the ciliated epithelial cells of nasal turbinates and infraorbital sinuses.

摘要

开发了一种免疫组织化学染色技术（IHC），使用链霉亲和素-生物素免疫过氧化物酶染色法在福尔马林固定、石蜡包埋的组织切片中检测禽肺病毒（APV）抗原。从实验接种APV的4周龄雏火鸡以及在自然发生的禽肺病毒疫情期间感染的成年火鸡中采集鼻甲和眶下窦样本。组织用10%缓冲中性福尔马林固定，石蜡包埋，切片并染色。在实验接种的雏火鸡和自然感染的禽类的鼻甲和眶下窦的黏膜和黏膜下层中，显微镜下观察到炎症变化。通过免疫组织化学在鼻甲和眶下窦的纤毛上皮细胞中检测到病毒抗原。