[DNA复制错误与结直肠癌临床病理特征的关系]

[The relation between DNA replication error and clinicopathological features of colorectal carcinoma].

作者信息

Xu N, Qiu H, Ding Y

机构信息

Department of Pathology, First Military Medical University, Guangzhou 510515.

出版信息

Zhonghua Bing Li Xue Za Zhi. 1998 Oct;27(5):359-61.

PMID:11245012

Abstract

OBJECTIVE

To study the relationship between DNA replication error and development of colorectal carcinoma (CRC).

METHODS

Silver staining PCR-SSCP and denatured polyacrylamide gel electrophoresis methods were used to detect microsatellites instability (MSI) at 4 loci on chromosome 2, 5, 17 in paraffin embedded specimens of 60 CRC and their paired normal tissue. If replication errors (RER) were found at 2 or more loci, then a diagnosis of RER+ was made.

RESULTS

RER+ was observed in 19/60 CRC, among which 7 cases had CRC family history. According to Amsterdam criteria, 4 were diagnosed as HNPCC(hereditary nonpolyposis colorectal cancer), of which 3 cases were RER+. The ratio of RER+ in HNPCC (75%) was significantly higher than that among sporadic CRC (28.5%). Most RER+ CRC have features of poorly differentiated adenocarcinoma (P < 0.01), tendency for right side involvement of the colon (P < 0.05) and a higher proportion with family history (P < 0.05). Rate of Dukes A and B stage higher than C and D stage (P < 0.05).

CONCLUSION

RER+ is a relatively common molecular event in CRC. RER+ CRC and RER- CRC have different clinicopathological features and behavior.

摘要

目的

研究DNA复制错误与结直肠癌（CRC）发生发展的关系。

方法

采用银染PCR-SSCP和变性聚丙烯酰胺凝胶电泳方法，检测60例CRC石蜡包埋标本及其配对正常组织中2号、5号、17号染色体上4个位点的微卫星不稳定性（MSI）。若在2个或更多位点发现复制错误（RER），则诊断为RER+。

结果

60例CRC中19例为RER+，其中7例有CRC家族史。根据阿姆斯特丹标准，4例诊断为HNPCC（遗传性非息肉病性结直肠癌），其中3例为RER+。HNPCC中RER+的比例（75%）显著高于散发性CRC（28.5%）。大多数RER+ CRC具有低分化腺癌特征（P<0.01）、结肠右侧受累倾向（P<0.05）以及家族史比例较高（P<0.05）。Dukes A和B期的比例高于C和D期（P<0.05）。