Jackowski G, Kacprzak K, Jansson S
Department of Plant Physiology, Adam Mickiewicz University, Poznañ, Poland.
Biochim Biophys Acta. 2001 Apr 2;1504(2-3):340-5. doi: 10.1016/s0005-2728(00)00262-0.
Using non-denaturing isoelectric focusing in polyacrylamide vertical slab gel, we have purified to homogeneity three trimeric subcomplexes of LHC II from Arabidopsis thylakoid membranes. The polypeptide composition of the subcomplexes were studied by immunoblotting. Our results indicate the existence in vivo of LHC II heterotrimers containing Lhcb1, Lhcb2 and Lhcb3 gene products.
利用非变性等电聚焦聚丙烯酰胺垂直平板凝胶,我们从拟南芥类囊体膜中纯化出了三种LHC II三聚体亚复合物,并使其达到了均一状态。通过免疫印迹法研究了这些亚复合物的多肽组成。我们的结果表明,在体内存在含有Lhcb1、Lhcb2和Lhcb3基因产物的LHC II异源三聚体。