Naqi S A, Panigrahy B, Hall C F
Am J Vet Res. 1975 Apr;36(4 Pt 2):548-52.
Purification and concentration of viruses contained in pathogenic material was accomplished by sucrose density gradient centrifugation. Electron microscopy was also performed. Viruses recovered from intestinal preparations of both affected and unaffected turkey poults had a density of 1.16 to 1.17. The viruses resembled myxoviruses, paramyxoviruses, or oncornaviruses morphologically. In addition, preparations from bluecomb-affected poults were found to contain viruses indistinguishable from known coronaviruses. No such virus particles were seen in the control preparations. Data are presented to indicate an etiologic relationship between the coronavirus and transmissible enteritis of turkeys.
通过蔗糖密度梯度离心法对致病物质中所含病毒进行纯化和浓缩。同时也进行了电子显微镜检查。从患病和未患病火鸡幼雏的肠道制剂中回收的病毒密度为1.16至1.17。这些病毒在形态上类似于黏液病毒、副黏病毒或肿瘤病毒。此外，发现来自患蓝冠病火鸡幼雏的制剂中含有与已知冠状病毒无法区分的病毒。在对照制剂中未见到此类病毒颗粒。所提供的数据表明冠状病毒与火鸡传染性肠炎之间存在病因学关系。
