Strik H M, Schluesener H J, Seid K, Meyermann R, Deininger M H
Institute of Brain Research, Medical School, University of Tuebingen, Germany.
Cancer. 2001 Mar 1;91(5):1013-9.
Endostatin is a potent inhibitor of endothelial cell proliferation, angiogenesis, and tumor growth. Its occurrence and localization has not yet been examined in human brain tumors. The authors report the production of a monoclonal antibody and detection of endostatin in rat and human gliomas by immunohistochemistry.
The authors analyzed localization and tissue distribution of endostatin in 41 paraffin embedded glioma samples (18 glioblastoma multiforme, 7 WHO Grade III astrocytomas, 13 fibrillary, and 3 protoplasmic WHO Grade II astrocytomas) of human origin and 21 rat C6 gliomas by immunohistochemistry. Double labeling experiments confirmed the origin of endostatin-labeled cells.
Endostatin immunoreactivity was detected in tumor cells, endothelial cells, macrophages, and lymphocytes of both rat and human gliomas. The percentage of cells labeled with the endostatin antibody was significantly lower (P = 0.0126) in the tumor parenchyma of human glioblastomas than in WHO Grade II astrocytomas.
Endostatin was present in various cell types in rat and human gliomas in vivo. Lower levels in glioblastomas than in WHO Grade II astrocytomas might have reflected the shift of a probable regulatory balance between promoters and inhibitors of angiogenesis towards facilitation of neovascularization.
内皮抑素是内皮细胞增殖、血管生成和肿瘤生长的有效抑制剂。其在人脑肿瘤中的发生和定位尚未得到研究。作者报告了一种单克隆抗体的产生以及通过免疫组织化学在大鼠和人类胶质瘤中检测内皮抑素的情况。
作者通过免疫组织化学分析了41例人源石蜡包埋胶质瘤样本（18例多形性胶质母细胞瘤、7例世界卫生组织III级星形细胞瘤、13例纤维型和3例原浆型世界卫生组织II级星形细胞瘤）以及21例大鼠C6胶质瘤中内皮抑素的定位和组织分布。双重标记实验证实了内皮抑素标记细胞的来源。
在大鼠和人类胶质瘤的肿瘤细胞、内皮细胞、巨噬细胞和淋巴细胞中均检测到内皮抑素免疫反应性。人胶质母细胞瘤肿瘤实质中被内皮抑素抗体标记的细胞百分比显著低于世界卫生组织II级星形细胞瘤（P = 0.0126）。
内皮抑素在大鼠和人类胶质瘤的多种细胞类型中均有存在。胶质母细胞瘤中内皮抑素水平低于世界卫生组织II级星形细胞瘤，这可能反映了血管生成促进因子和抑制因子之间可能的调节平衡向促进新生血管形成方向的转变。
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