[单层标本与传统涂片的比较]

[Comparison of monolayer specimens and conventional smears].

作者信息

Jensen M L, Fuursted P B, Svanholm H

机构信息

Randers Centralsygehus, patologisk institut.

出版信息

Ugeskr Laeger. 2001 Feb 26;163(9):1270-5.

PMID:11258251

Abstract

INTRODUCTION

Monolayer specimens were compared with conventional smears in a split sample study. The quality of the specimens, sensitivity, time consumption, and costs were elucidated.

METHOD

Conventional smears and fixated residual material from 1701 women were available.

RESULTS

The number of inadequate/less adequate specimens was reduced significantly (p < 0.001). Diagnostic agreement was found in 1531 (90%) of the 1701 cases (kappa = 0.52, SE (kappa) = 0.026). Dysplasia (NOS) was diagnosed in seven monolayer specimens (0.4%) versus 33 smears (1.9%) and ratio atypia/low grade was reduced by 33%, which indicates greater accuracy in diagnosis in monolayers. Histological follow-up showed sensitivities of 95% (monolayers) and 94% (smears). The time consumed (laboratory work, screening) on a smear versus a monolayer specimen was 11.5 minutes versus 9.3 minutes. Utensil costs of a smear are kr. 10.69. compared to kr. 26.50 for a monolayer specimen.

CONCLUSION

The higher costs should be set against the saving in significantly improved specimen quality, improved diagnostic accuracy, and shorter time consumption. The use of the monolayer technique (direct to vial) is recommended to replace the conventional smear.

摘要

引言

在一项样本分割研究中，将单层标本与传统涂片进行了比较。阐述了标本质量、敏感性、耗时及成本。

方法

有来自1701名女性的传统涂片和固定后的残余材料。

结果

不合格/不太合格标本的数量显著减少（p < 0.001）。1701例病例中有1531例（90%）诊断一致（kappa = 0.52，标准误（kappa）= 0.026）。在7个单层标本（0.4%）中诊断出发育异常（未另行说明），而在33份涂片中（1.9%）诊断出，非典型/低级别比例降低了33%，这表明单层标本诊断更准确。组织学随访显示敏感性分别为95%（单层标本）和94%（涂片）。一份涂片与一份单层标本的耗时（实验室工作、筛查）分别为11.5分钟和9.3分钟。一份涂片的器具成本为10.69瑞典克朗，而一份单层标本为26.50瑞典克朗。