Park J M, Gim B S, Kim J M, Yoon J H, Kim H S, Kang J G, Kim Y J
National Creative Research Initiative Center for Genome Regulation, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
Mol Cell Biol. 2001 Apr;21(7):2312-23. doi: 10.1128/MCB.21.7.2312-2323.2001.
To decipher the mechanistic roles of Mediator proteins in regulating developmental specific gene expression and compare them to those of TATA-binding protein (TBP)-associated factors (TAFs), we isolated and analyzed a multiprotein complex containing Drosophila Mediator (dMediator) homologs. dMediator interacts with several sequence-specific transcription factors and basal transcription machinery and is critical for activated transcription in response to diverse transcriptional activators. The requirement for dMediator did not depend on a specific core promoter organization. By contrast, TAFs are preferentially utilized by promoters having a specific core element organization. Therefore, Mediator proteins are suggested to act as a pivotal coactivator that integrates promoter-specific activation signals to the basal transcription machinery.
为了解析中介蛋白在调控发育特异性基因表达中的机制作用，并将其与TATA结合蛋白（TBP）相关因子（TAFs）的作用进行比较，我们分离并分析了一个包含果蝇中介体（dMediator）同源物的多蛋白复合物。dMediator与多种序列特异性转录因子和基础转录机制相互作用，对于响应多种转录激活因子的激活转录至关重要。对dMediator的需求并不依赖于特定的核心启动子结构。相比之下，TAFs优先被具有特定核心元件结构的启动子所利用。因此，中介蛋白被认为是一种关键的共激活因子，它将启动子特异性激活信号整合到基础转录机制中。
