Purification, crystallization and preliminary X-ray studies of thermostable alkaline phosphatase from Thermus sp. 3041.

作者信息

Ji C N, Jiang T, Chen M Q, Sheng X Y, Mao Y M

机构信息

Institute of Genetics, School of Life Science, Fudan University, Shanghai, People's Republic of China.

出版信息

Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):614-5. doi: 10.1107/s0907444901001226.

Thermostable alkaline phosphatase from Thermus sp. 3041 has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P2(1)22(1), with unit-cell parameters a = 57.7, b = 69.9, c = 111.5 A. Diffraction data were collected to 2.54 A with a completeness of 91.1% (87.8% for the last shell), an R(merge) value of 0.105 (0.312) and an I/sigma(I) value of 9.5 (3.6).