P53组织化学和DNA直方图参数在头颈部恶性肿瘤中的预后意义。

Prognostic significance of P53 histochemistry and DNA histogram parameters in head and neck malignancies.

作者信息

Tamás L, Kraxner H, Mechtler L, Répássy G, Ribári O, Hirschberg A, Szentkúti G, Járay B, Szentirmay Z

机构信息

Department of ENT, Jahn Ference Teaching Hospital, Semmelweis University of Medicine Affiliate, Köves u. 2-4., Budapest 1204, Hungary.

出版信息

Anticancer Res. 2000 Sep-Oct;20(5C):4031-7.

PMID:11268497

Abstract

BACKGROUND

The aim of the study was to determine the role of quantitative pathological parameters in prognosis of head and neck malignancies.

MATERIALS AND METHODS

51 head and neck squamous cell carcinoma patients were examined for mutant p53 gene expression (45 out of 51 patients) by immunohistochemistry and for cellular DNA-content (44 out of 51 patients) using digital picture analyzer. Statistical analysis was performed using BMDP package.

RESULTS

No correlation with prognosis was found for age, sex, localization, T-classification and therapy. There was significant relationship between N-status and overall survival (p = 0.0008). No correlation was found with overall and disease-free survival for either histologic type or grading. P53: No significant correlation was detected with overall survival. A relationship was found between mutant p53 and metastasis-free time (p = 0.06). Ploidy: There were no significant differences between aneuploid and euploid tumors for either disease-free or overall survival. Synthetic (S)-phase fraction: A correlation was found for both survival rates (p = 0.029) and metastasis-free time (p = 0.05). Polyploid fraction (PF): correlation was shown for both overall survival (p = 0.0128) and metastasis-free time (p = 0.0038).

CONCLUSION

There is correlation between p53 overexpression and metastatic potential and there is a significant relationship between SPF and PF value and prognosis (metastasis-free and overall survival) of head and neck cancer.

摘要

背景

本研究的目的是确定定量病理学参数在头颈部恶性肿瘤预后中的作用。

材料与方法

对51名头颈部鳞状细胞癌患者进行免疫组织化学检测突变型p53基因表达（51例患者中的45例），并使用数字图像分析仪检测细胞DNA含量（51例患者中的44例）。使用BMDP软件包进行统计分析。

结果

年龄、性别、肿瘤部位、T分期和治疗与预后均无相关性。N分期与总生存率之间存在显著关系（p = 0.0008）。组织学类型或分级与总生存率和无病生存率均无相关性。p53：与总生存率无显著相关性。发现突变型p53与无转移时间之间存在相关性（p = 0.06）。倍体：非整倍体肿瘤和整倍体肿瘤在无病生存率或总生存率方面均无显著差异。合成（S）期分数：与两种生存率（p = 0.029）和无转移时间（p = 0.05）均存在相关性。多倍体分数（PF）：与总生存率（p = 0.0128）和无转移时间（p = 0.0038）均存在相关性。