结核分枝杆菌对异烟肼的敏感性与katG基因第463位密码子的Arg→Leu突变无关。
The susceptibility of Mycobacterium tuberculosis to isoniazid and the Arg-->Leu mutation at codon 463 of katG are not associated.
作者信息
van Doorn H R, Kuijper E J, van der Ende A, Welten A G, van Soolingen D, de Haas P E, Dankert J
机构信息
Academic Medical Center, Department of Medical Microbiology, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 2001 Apr;39(4):1591-4. doi: 10.1128/JCM.39.4.1591-1594.2001.
Abstract
A mutation (CCG-->CTG [Arg-->Leu]) in codon 463 of katG (catalase peroxidase) of Mycobacterium tuberculosis has been found in isoniazid (INH)-resistant strains. A PCR restriction endonuclease analysis to detect this mutation was applied to 395 M. tuberculosis isolates from patients in The Netherlands. The proportion of isolates with a detectable mutation was 32% (32 out of 100) and 29% (85 out of 295) among INH-susceptible isolates and INH-resistant or -intermediate isolates, respectively. Sequencing of five INH-susceptible isolates with such mutations showed that all five had the Arg463Leu mutation. We conclude that the Arg463Leu mutation of katG of M. tuberculosis is not a reliable indicator of INH resistance.
摘要
在耐异烟肼（INH）的结核分枝杆菌菌株中发现了katG（过氧化氢酶过氧化物酶）第463位密码子的突变（CCG→CTG [精氨酸→亮氨酸]）。一种用于检测该突变的聚合酶链反应-限制性内切酶分析方法应用于来自荷兰患者的395株结核分枝杆菌分离株。在INH敏感分离株和INH耐药或中介分离株中，可检测到突变的分离株比例分别为32%（100株中的32株）和29%（295株中的85株）。对五株具有此类突变的INH敏感分离株进行测序表明，所有五株均具有Arg463Leu突变。我们得出结论，结核分枝杆菌katG的Arg463Leu突变不是INH耐药性的可靠指标。
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