核酸修复在功能基因组学中的潜力。

The potential of nucleic acid repair in functional genomics.

作者信息

Rice M C, Czymmek K, Kmiec E B

机构信息

Department of Biological Science, University of Delaware, Newark, DE 19716, USA.

出版信息

Nat Biotechnol. 2001 Apr;19(4):321-6. doi: 10.1038/86701.

DOI:10.1038/86701

PMID:11283588

Abstract

Chimeric RNA/DNA oligonucleotides have been used successfully to correct point and frameshift mutations in cells as well as in animal and plant models. This approach is one of several nucleic acid repair technologies that will help elucidate the function of newly discovered genes. Understanding the mechanisms by which these different technologies direct gene alteration is essential for progress in their application to functional genomics.

摘要

嵌合RNA/DNA寡核苷酸已成功用于纠正细胞以及动植物模型中的点突变和移码突变。这种方法是几种核酸修复技术之一，将有助于阐明新发现基因的功能。了解这些不同技术指导基因改变的机制对于它们在功能基因组学中的应用进展至关重要。