Bcl-2表达作为非小细胞肺癌的凋亡指标。

Bcl-2 expression as an apoptotic index in non small cell lung carcinomas.

作者信息

Kalogeraki A, Kozoni V, Zoras O, Tamiolakis D, Panayiotides J, Charakidas A, Chalkiadakis G, Bouros D, Stathopoulos E, Delides G S

机构信息

Department of Pathology, Medical School, University of Crete, P.O. Box 1393, TT 71110, Heraklion, Crete, Greece.

出版信息

In Vivo. 2001 Jan-Feb;15(1):53-6.

PMID:11286130

Abstract

To evaluate the bcl-2 protein expression in non small cell lung carcinomas (NSCLC) as an index of apoptosis of these tumors, in fine needle aspiration biopsies (FNABs) of the lung, we studied thirty-eight cases of NSCLC (25 bronchogenic adenocarcinomas and 13 squamous. carcinomas. Bcl-2 protein was used as the primary antibody (monoclonal, DAKO) by Alkaline-phosphatase method. Very light haematoxylin was performed as the counterstain. The results were compared and confirmed histologically. A cytoplasmatic expression of the bcl-2 protein was found in 72% (18 out of 15) of the bronchogenic adenocarcinomas while 61.54% (8 out of 13) of squamous carcinomas showed bcl-2 expression. For the quantitative analysis of our results, we used the t-test and the difference between those two histologic types was regarded as statistically significant with p < 0.001.

摘要

为了评估非小细胞肺癌（NSCLC）中bcl-2蛋白的表达情况，以此作为这些肿瘤细胞凋亡的一个指标，我们在肺细针穿刺活检（FNAB）中，研究了38例非小细胞肺癌（25例支气管源性腺癌和13例鳞癌）。采用碱性磷酸酶法，将bcl-2蛋白作为一抗（单克隆抗体，DAKO）。用极淡的苏木精进行复染。将结果进行比较并通过组织学检查加以证实。在支气管源性腺癌中，72%（15例中的18例）发现有bcl-2蛋白的细胞质表达，而在鳞癌中，61.54%（13例中的8例）显示有bcl-2表达。为了对我们的结果进行定量分析，我们采用了t检验，这两种组织学类型之间的差异被认为具有统计学意义，p < 0.001。