d(pT)-10-n寡核苷酸的制备与分离
Preparation and separation of d(pT)-10-n oligonucleotides.
作者信息
Raae A J, Lillehaug J R, Kleppe R K, Kleppe K
出版信息
Nucleic Acids Res. 1975 Mar;2(3):423-9. doi: 10.1093/nar/2.3.423.
Abstract
A series of oligomers having the general formula d(pT)-10-n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)-10, annealed on poly dA, employing T-4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and initiators for various polymerases.
摘要
通过使用T-4多核苷酸连接酶，将d(pT)-10在聚dA上退火后进行酶促连接，制备了一系列通式为d(pT)-10-n（n从2到20不等）的寡聚物。这些寡聚物可在8%或12%的聚丙烯酰胺凝胶上分离。此类寡聚物可能被证明可作为各种聚合酶的分子量标记物和引发剂。
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