Fleeton M N, Chen M, Berglund P, Rhodes G, Parker S E, Murphy M, Atkins G J, Liljeström P
Microbiology and Tumorbiology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden.
J Infect Dis. 2001 May 1;183(9):1395-8. doi: 10.1086/319857. Epub 2001 Mar 30.
In genetic vaccination, recipients are immunized with antigen-encoding nucleic acid, usually DNA. This study addressed the possibility of using the recombinant alpha virus RNA molecule, which replicates in the cytoplasm of transfected cells, as a novel approach for genetic vaccination. Mice were immunized with recombinant Semliki Forest virus RNA-encoding envelope proteins from one of 3 viruses: influenza A virus, a tickborne flavivirus (louping ill virus), or respiratory syncytial virus (RSV). Serologic analyses showed that antigen-specific antibody responses were elicited. IgG isotyping indicated that predominantly Th1 type immune responses were induced after immunization with RSV F protein-encoding RNA, which is relevant for protection against RSV infection. Challenge infection showed that RNA immunization had elicited significant levels of protection against the 3 model virus diseases.
在基因疫苗接种中，接受者用编码抗原的核酸（通常是DNA）进行免疫。本研究探讨了使用在转染细胞胞质中复制的重组α病毒RNA分子作为基因疫苗接种新方法的可能性。用编码来自三种病毒之一的包膜蛋白的重组塞姆利基森林病毒RNA对小鼠进行免疫：甲型流感病毒、一种蜱传黄病毒（卢平病病毒）或呼吸道合胞病毒（RSV）。血清学分析表明引发了抗原特异性抗体反应。IgG亚型分析表明，用编码RSV F蛋白的RNA免疫后主要诱导了Th1型免疫反应，这与预防RSV感染相关。攻毒感染表明RNA免疫引发了针对这三种模型病毒疾病的显著保护水平。
