（A + T）-富含区结合蛋白对麻蝇凝集素基因启动子的激活作用。

Activation of the Sarcophaga lectin gene promoter by (A + T)-stretch binding protein.

作者信息

Aozasa N, Shiraishi H, Nakanishi-Matsui M, Kobayashi A, Sekimizu K, Kubo T, Natori S

机构信息

Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0033; Natori Special Laboratory, The Institute of Physical and Chemical Research, Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan.

出版信息

Eur J Biochem. 2001 Apr;268(8):2506-11. doi: 10.1046/j.1432-1327.2001.02133.x.

DOI:10.1046/j.1432-1327.2001.02133.x

PMID:11298770

Abstract

Previously, we purified and isolated a cDNA for (A + T)-stretch binding protein (ATBP) that binds to (A + T)-stretches in the 5' upstream region of the Sarcophaga lectin gene [Nakanishi-Matsui, M., Kubo, T. & Natori, S. (1995) Eur. J. Biochem. 230, 396-400]. Here, we used a luciferase reporter to examine the effect of ATBP on transcription of the Sarcophaga lectin gene. Deletion experiments revealed that ATBP activates the Sarcophaga lectin gene in a 5' upstream sequence-dependent manner, and that at least the N-terminal 25 residues, the three Zn-finger domains, an acidic domain and the third hydrophobic domain of ATBP are indispensable for its function. Furthermore, a synergistic effect was detected between ATBP and Dif, suggesting that ATBP is involved in the activation of insect immunity genes.

摘要

此前，我们纯化并分离出了一种与（A + T）重复序列结合蛋白（ATBP）的cDNA，该蛋白可与肉蝇凝集素基因5'上游区域的（A + T）重复序列结合[Nakanishi-Matsui, M., Kubo, T. & Natori, S. (1995) Eur. J. Biochem. 230, 396 - 400]。在此，我们使用荧光素酶报告基因来检测ATBP对肉蝇凝集素基因转录的影响。缺失实验表明，ATBP以5'上游序列依赖的方式激活肉蝇凝集素基因，并且ATBP至少其N端的25个残基、三个锌指结构域、一个酸性结构域和第三个疏水结构域对其功能是不可或缺的。此外，还检测到ATBP与Dif之间存在协同效应，这表明ATBP参与了昆虫免疫基因的激活。