来自牛乳的球三糖神经酰胺（Gb3）对大肠杆菌O157:H7裂解物中Vero细胞细胞毒性活性的抑制作用。

Inhibition of Vero cell cytotoxic activity in Escherichia coli O157:H7 lysates by globotriaosylceramide, Gb3, from bovine milk.

作者信息

Watarai S, Inoue K, Kushi Y, Isogai E, Yokota K, Naka K, Oguma K, Kodama H

机构信息

Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan.

出版信息

Biosci Biotechnol Biochem. 2001 Feb;65(2):414-9. doi: 10.1271/bbb.65.414.

DOI:10.1271/bbb.65.414

PMID:11302178

Abstract

In order to clarify the presence and verotoxin (VT) inhibitory activity of globotriaosylceramide (Gb3) in bovine milk, we analyzed neutral glycosphingolipids (GSLs) from bovine milk and investigated the inhibitory effect of bovine milk Gb3 on the cytotoxicity of VT2. Five species of neutral GSLs, designated as N-1, N-2, N-3, N-4, and N-5, were separated on thin-layer chromatography (TLC). N-1, N-2, and N-3 showed the same mobility as glucosylceramide, lactosylceramide, and Gb3 on the TLC plate, respectively. N-4 and N-5 GSLs migrated below globoside on the TLC plate. N-3 GSL having the same TLC mobility as Gb3 from bovine milk was immunologically identified as Gb3 by monoclonal antibody against Gb3, anti-CD77 monoclonal antibody. Furthermore, the effect of bovine milk Gb3 on VT2-induced cytotoxicity was investigated. We found that treatment of VT2 with bovine milk Gb3 can reduce the cytotoxic effect of VT2.

摘要

为了阐明牛乳中球三糖神经酰胺（Gb3）的存在及其对志贺毒素（VT）的抑制活性，我们分析了牛乳中的中性糖鞘脂（GSLs），并研究了牛乳Gb3对VT2细胞毒性的抑制作用。通过薄层色谱（TLC）分离出了5种中性GSLs，分别命名为N-1、N-2、N-3、N-4和N-5。在TLC板上，N-1、N-2和N-3分别与葡萄糖神经酰胺、乳糖神经酰胺和Gb3具有相同的迁移率。N-4和N-5 GSLs在TLC板上的迁移位置低于糖苷脂。通过抗Gb3单克隆抗体、抗CD77单克隆抗体免疫鉴定出与牛乳Gb3具有相同TLC迁移率的N-3 GSLs为Gb3。此外，还研究了牛乳Gb3对VT2诱导的细胞毒性的影响。我们发现，用牛乳Gb3处理VT2可以降低VT2的细胞毒性作用。