Hase C, Nievergelt J, Moënne-Loccoz Y, Défago G
Phytopathology Group, Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland.
J Appl Microbiol. 2001 Apr;90(4):567-77. doi: 10.1046/j.1365-2672.2001.01280.x.
To assess the effects of soil type and time of the year on survival of the biocontrol inoculant Pseudomonas fluorescens CHA0 under aerobic conditions in lysimeter effluent water.
Effluent water was collected at different times from large outdoor lysimeters (2.5 m deep), which contained a well-drained or a poorly-drained cambisol, and inoculated with CHA0. The inoculant was monitored for 175 d by colony counts, total immunofluorescence cell counts and Kogure's viable cell counts. Cell numbers obtained with the three methods were similar. The inoculant declined exponentially in time and its population level varied considerably depending on the time of the year at which effluent water had been collected and soil type in the lysimeter. Positive correlations were found between the number of resident culturable aerobic bacteria and subsequent survival of the inoculant.
The fluctuations of inoculant survival patterns correlated with differences in biological properties of lysimeter water that were related to soil type and time of the year.
Results suggest that predictability of the survival of bacterial soil inoculants transported to groundwater level by heavy rainfall may be improved by taking into account key biological properties of the water.
评估土壤类型和一年中的时间对生物防治接种剂荧光假单胞菌CHA0在有氧条件下在蒸渗仪流出水中存活的影响。
在不同时间从大型室外蒸渗仪（2.5米深）收集流出水，这些蒸渗仪包含排水良好或排水不良的始成土，并接种CHA0。通过菌落计数、总免疫荧光细胞计数和小暮氏活菌计数对接种剂进行175天的监测。用这三种方法获得的细胞数量相似。接种剂数量随时间呈指数下降，其种群水平根据收集流出水的一年中的时间和蒸渗仪中的土壤类型而有很大差异。发现常驻可培养需氧细菌数量与接种剂随后的存活之间存在正相关。
接种剂存活模式的波动与蒸渗仪水的生物学特性差异相关，这些差异与土壤类型和一年中的时间有关。
结果表明，通过考虑水的关键生物学特性，可能会提高对因暴雨输送到地下水位的细菌土壤接种剂存活的可预测性。
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