Kotchen T A, Talwalkar R T, Kotchen J M, Miller M C, Welch W J
Circ Res. 1975 Jun;36(6 Suppl 1):17-27. doi: 10.1161/01.res.36.6.17.
After addition of exogenous human renin, the in vitro rate of angiotensin I generation is faster in plasma of patients with chronic renal insufficiency and, to a lesser extent, in plasma of patients with essential hypertension than in plasma from normotensive control subjects. The increased reactivity of renin in hypertensive and uremic plasma is not related to differences of endogenous renin activity, angiotensinase activity, renin substrate concentration, or substrate reactivity. Addition of normal, hypertensive, and uremic plasma to a human renin-sheep renin substrate system inhibited the rate of angiotensin generation, although significantly less inhibition was observed with uremic plasma. The reactivity of renin increased in normal plasma but not in uremic plasma after treatment with 95% acetone. After acetone extraction renin reactivity in normal and plasma inhibited the rate of angiotensin generation in a renin-renin substrate system. Less inhibition occurred with the acetone extract from a pool of uremic plasma. These results provide evidence for the existence of a naturally occurring acetone soluble renin inhibiting factor in normal and uremic plasma. The increased reactivity of renin in uremic plasma may be related to a deficiency of this factor.
加入外源性人肾素后，慢性肾功能不全患者血浆中血管紧张素I的体外生成速率比正常血压对照受试者的血浆更快，原发性高血压患者血浆中的生成速率相对较慢。高血压和尿毒症血浆中肾素反应性的增加与内源性肾素活性、血管紧张素酶活性、肾素底物浓度或底物反应性的差异无关。将正常、高血压和尿毒症血浆加入人肾素 - 羊肾素底物系统中会抑制血管紧张素的生成速率，不过尿毒症血浆的抑制作用明显较小。用95%丙酮处理后，正常血浆中肾素的反应性增加，但尿毒症血浆中肾素的反应性未增加。丙酮提取后，正常血浆和尿毒症血浆中的肾素反应性在肾素 - 肾素底物系统中抑制了血管紧张素的生成速率。尿毒症血浆池的丙酮提取物产生的抑制作用较小。这些结果证明正常和尿毒症血浆中存在一种天然存在的丙酮可溶性肾素抑制因子。尿毒症血浆中肾素反应性的增加可能与该因子的缺乏有关。
