利用最近建立的双色荧光原位杂交技术检测上皮性卵巢癌中17号染色体的数目畸变及c-erbB2基因扩增。

Detection of numerical aberration in chromosome 17 and c-erbB2 gene amplification in epithelial ovarian cancer using recently established dual color FISH.

作者信息

Fukushi Y, Sato S, Yokoyama Y, Kudo K, Maruyama H, Saito Y

机构信息

Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Japan.

出版信息

Eur J Gynaecol Oncol. 2001;22(1):23-5.

PMID:11321488

Abstract

This paper presents a new dual color FISH technique that will allow the use of cell specimens isolated from formaldehyde-fixed and paraffin-embedded (F-P) tissues. The new FISH method was used to examine numerical aberration in chromosome 17 and c-erbB2 gene amplification in 26 F-P epithelial ovarian cancer tissues. Numerical aberration of chromosome 17 appears frequently in clear cell adenocarcinoma. The frequency of amplification of the c-erbB2 gene in stage III and IV cases was significantly higher than in stage I cases. The FISH technique as established here may serve as a molecular tool for examination of clinico-pathological significance in F-P tissues.

摘要

本文介绍了一种新的双色荧光原位杂交（FISH）技术，该技术可用于检测从甲醛固定石蜡包埋（F-P）组织中分离出的细胞标本。采用这种新的FISH方法检测了26例F-P上皮性卵巢癌组织中17号染色体的数目畸变及c-erbB2基因扩增情况。17号染色体数目畸变在透明细胞腺癌中较为常见。Ⅲ期和Ⅳ期病例中c-erbB2基因扩增频率显著高于Ⅰ期病例。本文建立的FISH技术可作为检测F-P组织临床病理意义的分子工具。