Reischl U, Lehn N, Sanden G N, Loeffelholz M J
Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany.
J Clin Microbiol. 2001 May;39(5):1963-6. doi: 10.1128/JCM.39.5.1963-1966.2001.
Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.
据报道,通过针对百日咳博德特氏菌IS481的实时PCR检测法检测霍氏博德特氏菌。对来自百日咳博德特氏菌和霍氏博德特氏菌分离株的IS481特异性PCR产物进行测序,结果显示序列同源性。限制性片段长度多态性表明霍氏博德特氏菌中IS481样序列的拷贝数较低。这些结果,以及从咳嗽患者中培养出霍氏博德特氏菌,提示基于IS481的百日咳PCR检测法的特异性和预测价值可能受到影响。