神经元限制性沉默因子可作为神经元细胞中发动蛋白I基因启动子活性的激活因子。
The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells.
作者信息
Yoo J, Jeong M J, Lee S S, Lee K I, Kwon B M, Kim D S, Park Y M, Han M Y
机构信息
Cell Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, 305-600, Korea.
出版信息
Biochem Biophys Res Commun. 2001 May 18;283(4):928-32. doi: 10.1006/bbrc.2001.4857.
The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-kappaB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.
神经元限制性沉默元件（NRSE）已在多个神经元基因中被鉴定出来，它通过在非神经元细胞中沉默转录来赋予神经元特异性。我们之前报道过，Sp1和一个类NF-κB元件（NE-1）是小鼠发动蛋白I基因启动子活性所必需的。在本研究中，我们发现发动蛋白I启动子的上游调控区域有一个类似NRSE的序列，并表明神经元限制性沉默因子（NRSF）在神经元细胞和非神经元细胞中均能与该元件结合。我们还表明，NRSF在神经元细胞中激活发动蛋白I基因的启动子活性。根据本研究结果，我们推测NRSE可能参与发动蛋白I表达的神经元限制性调控，并且NRSF可能在神经元细胞中作为发动蛋白I基因启动子活性的激活剂发挥作用。
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