γ射线显著抑制动脉平滑肌细胞对水合胶原凝胶的收缩作用。

Gamma-irradiation markedly inhibits the hydrated collagen gel contradiction by arterial smooth muscle cells.

作者信息

Keller P F, Verin V, Ziegler T, Mermillod B, Popowski Y, Delafontaine P

机构信息

Department of Cardiology, University Hospital, Geneva, Switzerland.

出版信息

J Investig Med. 2001 May;49(3):258-64. doi: 10.2310/6650.2001.33970.

DOI:10.2310/6650.2001.33970

PMID:11352183

Abstract

BACKGROUND

Vessel wall responses to percutaneous transluminal coronary angioplasty include neointimal proliferation and arterial remodeling. The contraction of a collagen gel is a good in vitro model of wound repair and vascular remodeling. Because irradiation is an important new therapeutic modality capable of preventing restenosis, the purpose of this study was to evaluate the effect of irradiation on the contraction of a collagen gel by smooth muscle cells (SMCs), on SMCs viability, and on DNA synthesis.

METHODS

We studied the effect of different doses of gamma-irradiation (0 [control], 6, 12, and 18 Gy) on the contraction of a collagen gel seeded with SMCs (calf carotid arteries) during a period of 15 days.

RESULTS

Maximal gel diameter reduction (from 35 to 6.8 mm, +/-0.5 mm in control) was markedly inhibited in the 6-, 12-, and 18-Gy groups (35 to 13.7 mm, +/-0.8 mm; 35 to 15.5 mm, +/-0.9 mm; and 35 to 16.1 mm, +/-0.9 mm, respectively; P<0.0001). The irradiated gels showed a dose-dependent reduction in the SMC proliferation rate (P<0.0001) and an increase in the number of nonviable SMCs (P<0.002) 15 days after irradiation.

CONCLUSIONS

Gamma-irradiation produces a significant dose-dependent inhibition of the contraction of collagen gels seeded with arterial SMCs. This effect is related to a significant decrease in SMC viability and a decrease in SMC proliferation rate. These findings shed light on mechanisms whereby irradiation may positively affect arterial remodeling after percutaneous transluminal coronary angioplasties.

摘要

背景

血管壁对经皮腔内冠状动脉成形术的反应包括新生内膜增殖和动脉重塑。胶原凝胶的收缩是伤口修复和血管重塑的良好体外模型。由于放射治疗是一种能够预防再狭窄的重要新治疗方式，本研究的目的是评估放射对平滑肌细胞（SMC）引起的胶原凝胶收缩、SMC活力以及DNA合成的影响。

方法

我们研究了不同剂量的γ射线照射（0[对照]、6、12和18 Gy）对接种SMC（小牛颈动脉）的胶原凝胶在15天内收缩的影响。

结果

6 Gy、12 Gy和18 Gy组的凝胶最大直径减少（对照为35至6.8 mm，±0.5 mm）受到显著抑制（分别为35至13.7 mm，±0.8 mm；35至15.5 mm，±0.9 mm；35至16.1 mm，±0.9 mm；P<0.0001）。照射后15天，照射的凝胶显示SMC增殖率呈剂量依赖性降低（P<0.0001），非存活SMC数量增加（P<0.002）。