Nakanishi K, Kawai T, Aida S, Kasamatsu H, Aurues T, Ikeda T
Division of Environmental Medicine, National Defense Medical College Research Institute, Tokorozawa, Japan.
Mod Pathol. 2001 May;14(5):371-6. doi: 10.1038/modpathol.3880320.
The expression of p27(Kip1), a negative regulator of the cell cycle, has been reported to correlate with the biological behavior and prognosis of several tumors. However, its prognostic importance in transitional cell carcinoma of the upper urinary tract (TCC-UUT) has not previously been investigated. We investigated p27(Kip1) protein expression using immunohistochemistry in 132 cases of TCC-UUT and also its relation to proliferating cell nuclear antigen (PCNA) immunoreactivity, p53 oncoprotein immunoreactivity, clinicopathologic parameters, and clinical outcome. A positive expression of p27(Kip1) protein was recognized in 94.7% of the samples and was apparent within tumor nuclei. In the normal urothelium, its expression was identified in all cell layers. A positive expression of p53 oncoprotein was recognized in 27.2% of the patients. The PCNA index was 7.4 to 93.1% (mean, 66.4%). Examination of the relationships between the expression of p27(Kip1) protein and clinicopathologic findings, PCNA index, and the expression of p53 oncoprotein revealed that the expression of p27(Kip1) protein decreased significantly with stage and grade. In a univariate analysis of disease-free and overall survival rates, no correlation was found between the expression of p27(Kip1) protein and prognosis. The expression of p27(Kip1) protein appears to be of little or no value in informing the prognosis in TCC-UUT.
细胞周期负调控因子p27（Kip1）的表达已被报道与多种肿瘤的生物学行为和预后相关。然而，其在上尿路移行细胞癌（TCC-UUT）中的预后重要性此前尚未得到研究。我们采用免疫组织化学方法检测了132例TCC-UUT中p27（Kip1）蛋白的表达，并研究了其与增殖细胞核抗原（PCNA）免疫反应性、p53癌蛋白免疫反应性、临床病理参数及临床结局的关系。94.7%的样本中可识别出p27（Kip1）蛋白的阳性表达，且在肿瘤细胞核内明显可见。在正常尿路上皮中，其表达见于所有细胞层。27.2%的患者中可识别出p53癌蛋白的阳性表达。PCNA指数为7.4%至93.1%（平均66.4%）。对p27（Kip1）蛋白表达与临床病理结果、PCNA指数及p53癌蛋白表达之间关系的研究显示，p27（Kip1）蛋白的表达随分期和分级显著降低。在无病生存率和总生存率的单因素分析中，未发现p27（Kip1）蛋白表达与预后之间存在相关性。p27（Kip1）蛋白的表达在上尿路移行细胞癌的预后判断中似乎价值不大或没有价值。
