Kim S H, Song S H, Kim Y J, Park S Y
Antibody Engineering Laboratory, Central Research Center of the Korea Green Cross Corporation, Yongin, Korea.
Mol Cells. 2001 Apr 30;11(2):158-63.
Alpha-fetoprotein (AFP) is a well-known molecular marker indicating the development of cancer as well as fetal abnormalities such as open neural tube defects. Accordingly the measurement of serum AFP is important for the diagnosis of hepatocellular carcinoma (HCC) and other abnormalities. Monoclonal antibodies (McAb) to AFP were produced to develop an immunoassay kit, and to study the possibility of an antibody (Ab) therapy. The immunoglobulin genes were cloned from hybridoma cells, and expressed in E. coli as an Fab soluble into a culture medium. The Fab of anti-AFP McAb exhibited binding to AFP and similar affinity compared to the original IgG. This recombinant antibody can be studied further for in vivo imaging and immunotherapeutics.
甲胎蛋白（AFP）是一种著名的分子标志物，可指示癌症的发展以及胎儿异常情况，如开放性神经管缺陷。因此，血清AFP的检测对于肝细胞癌（HCC）和其他异常情况的诊断至关重要。为了开发免疫测定试剂盒并研究抗体治疗的可能性，制备了针对AFP的单克隆抗体（McAb）。从杂交瘤细胞中克隆了免疫球蛋白基因，并在大肠杆菌中表达为可溶于培养基的Fab片段。抗AFP McAb的Fab片段与AFP表现出结合能力，且与原始IgG相比具有相似的亲和力。这种重组抗体可进一步用于体内成像和免疫治疗研究。
