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I型毛发角蛋白在毛囊肿瘤中的表达

Expression of type I hair keratins in follicular tumours.

作者信息

Cribier B, Peltre B, Langbein L, Winter H, Schweizer J, Grosshans E

机构信息

Laboratory of Cutaneous Histopathology, University Hospital, 1 Place de l'Hôpital, 67091 Strasbourg, France.

出版信息

Br J Dermatol. 2001 May;144(5):977-82. doi: 10.1046/j.1365-2133.2001.04185.x.

Abstract

BACKGROUND

Hair keratins are specifically expressed in hair and nails. We previously demonstrated the expression of hair keratin basic 1 mRNA in pilomatrixomas. We recently developed a method for immunohistochemical staining of the group of acidic keratins, which have not yet been investigated in human tumours.

OBJECTIVES

To study the expression of eight members of the type I hair keratin subfamily in pilomatrixomas and other skin tumours of follicular origin.

METHODS

We performed immunohistochemistry on paraffin sections of formalin-fixed pilomatrixomas (40), trichoepitheliomas (10), trichoblastomas (10), desmoplastic trichoepitheliomas (10) and basal cell carcinomas (10), using antibodies against type I hair keratins hHa1, hHa2, hHa3-II, hHa4, hHa5, hHa6, hHa7 and hHa8 as well as cytokeratin CK17.

RESULTS

While CK17 was found in almost all tumours investigated, hair keratins were exclusively expressed in pilomatrixomas. Their expression was restricted to areas of transitional cells, located between outer basophilic matricial cells and an inner zone of eosinophilic shadow cells. The most frequently and most strongly expressed hair keratins were hHa1, hHa2, hHa5 and hHa8, whereas hHa4 and hHa6 were only weakly expressed. No positive staining was observed with anti-hHa3-II and anti-hHa7 antibodies. Hair keratin expression in intermediate maturation stage pilomatrixomas resembled that of normal hair follicles, with early matricial and cuticular keratins hHa5 and hHa2 being expressed in lower transitional cells, followed by expression of early cortex keratins hHa1 and hHa8 in intermediate transitional cells and the late cortex keratins hHa4 and hHa6 in upper transitional cells. The latter were, however, seen only in a few intermediate maturation stage pilomatrixomas and were generally absent in late-stage pilomatrixomas.

CONCLUSIONS

These changes in hair keratin expression patterns indicate that the maturation of pilomatrixomas towards large areas of shadow cells is associated with a gradual loss of differentiation-specific hair keratins. The complex hair keratin expression in pilomatrixomas is a further argument in favour of a hair matrix origin of this tumour.

摘要

背景

毛发角蛋白在毛发和指甲中特异性表达。我们之前已证实毛发角蛋白碱性1 mRNA在毛母质瘤中的表达。我们最近开发了一种针对酸性角蛋白组的免疫组织化学染色方法,该酸性角蛋白组尚未在人类肿瘤中进行研究。

目的

研究I型毛发角蛋白亚家族的八个成员在毛母质瘤和其他毛囊源性皮肤肿瘤中的表达。

方法

我们使用针对I型毛发角蛋白hHa1、hHa2、hHa3-II、hHa4、hHa5、hHa6、hHa7和hHa8以及细胞角蛋白CK17的抗体,对福尔马林固定的毛母质瘤(40例)、毛发上皮瘤(10例)、毛母细胞瘤(10例)、促结缔组织增生性毛发上皮瘤(10例)和基底细胞癌(10例)的石蜡切片进行免疫组织化学检测。

结果

虽然在几乎所有研究的肿瘤中都发现了CK17,但毛发角蛋白仅在毛母质瘤中表达。它们的表达局限于位于嗜碱性外层基质细胞和嗜酸性影子细胞内层之间的过渡细胞区域。最常且最强表达的毛发角蛋白是hHa1、hHa2、hHa5和hHa8,而hHa4和hHa6仅弱表达。抗hHa3-II和抗hHa7抗体未观察到阳性染色。中间成熟阶段毛母质瘤中的毛发角蛋白表达类似于正常毛囊,早期基质和角质层角蛋白hHa5和hHa2在较低的过渡细胞中表达,随后早期皮质角蛋白hHa1和hHa8在中间过渡细胞中表达,晚期皮质角蛋白hHa4和hHa6在上层过渡细胞中表达。然而,后者仅在少数中间成熟阶段的毛母质瘤中可见,在晚期毛母质瘤中通常不存在。

结论

毛发角蛋白表达模式的这些变化表明,毛母质瘤向大面积影子细胞的成熟与分化特异性毛发角蛋白的逐渐丧失有关。毛母质瘤中复杂的毛发角蛋白表达进一步支持了该肿瘤起源于毛发基质的观点。

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