106例肺癌中P16和Rb蛋白的表达

[The expression of P16 and Rb proteins in 106 cases of lung cancer].

作者信息

Qin J, Chen G, Wang X

机构信息

Thoracic Department, Tianjin Thoracic Hospital, Tianjin 300051, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2000 Oct;23(10):588-90.

PMID:11372380

Abstract

OBJECTIVE

To observe the relationship between the expression of P16 and Rb proteins and the proliferation of lung cancer cells.

METHODS

The expressions of P16 and Rb proteins were measured in 66 cases of NSCLC and 40 cases of SCLC by means of immunohistochemistry technique.

RESULTS

The inverse correlation of Rb and P16 proteins expression was confirmed in 66 cases of NSCLC (chi 2 = 10.52, P < 0.01). No relationship between the expression of P16 and differentiation, stage, lymph node metastasis or pathological type was found (P > 0.05). There was no correlation between the expression of Rb and differentiation, stage, lymph node metastasis or pathological pattern (P > 0.05). The frequency of the loss of Rb protein expression in SCLC (78%) was significantly higher than in NSCLC (21%, chi 2 = 32.3, P < 0.01).

CONCLUSIONS

Loss of Rb protein expression in SCLC may be an important marker of the diagnosis of SCLC. The expression of P16 adversely correlates with that of Rb. This study provides strong confirmation about that there might be a negative feedback between P16 and Rb in phase G1.

摘要

目的

观察P16和Rb蛋白表达与肺癌细胞增殖之间的关系。

方法

采用免疫组织化学技术检测66例非小细胞肺癌（NSCLC）和40例小细胞肺癌（SCLC）中P16和Rb蛋白的表达。

结果

66例NSCLC中Rb和P16蛋白表达呈负相关（χ2 = 10.52，P < 0.01）。未发现P16表达与分化、分期、淋巴结转移或病理类型之间存在相关性（P > 0.05）。Rb表达与分化、分期、淋巴结转移或病理类型之间无相关性（P > 0.05）。SCLC中Rb蛋白表达缺失的频率（78%）显著高于NSCLC（21%，χ2 = 32.3，P < 0.01）。