采用紫外诱导荧光检测的毛细管等电聚焦法。

Capillary isoelectric focusing with UV-induced fluorescence detection.

作者信息

Horká M, Willimann T, Blum M, Nording P, Friedl Z, Slais K

机构信息

Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno.

出版信息

J Chromatogr A. 2001 May 4;916(1-2):65-71. doi: 10.1016/s0021-9673(00)01069-4.

DOI:10.1016/s0021-9673(00)01069-4

PMID:11382311

Abstract

Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV photometric and UV excited fluorometric detection. The experimental set-up of capillary IEF with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented.

摘要

具有合适酸碱和电泳性质、在近紫外区域可激发的低分子质量荧光化合物被建议用作等电聚焦（IEF）的等电点（pI）标记物，用于紫外光度检测和紫外激发荧光检测。给出了具有紫外激发荧光检测的毛细管IEF实验装置以及新型紫外诱导荧光pI标记物的性质。通过与IEF方法无关的方式测定的18种新型pI标记物的pI值范围为2.1至10.3。展示了通过具有光度或荧光检测的毛细管IEF分离新型pI标记物以及衍生化蛋白质的实例。