采用毛细管区带电泳法对水果和菠菜中的L-抗坏血酸及总抗坏血酸进行定量分析。

Quantification of L-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis.

作者信息

Liao T, Jiang C M, Wu M C, Hwang J Y, Chang H M

机构信息

Graduate Institute of Food Science and Technology, National Taiwan University, Taipei.

出版信息

Electrophoresis. 2001 May;22(8):1484-8. doi: 10.1002/1522-2683(200105)22:8<1484::AID-ELPS1484>3.0.CO;2-9.

DOI:10.1002/1522-2683(200105)22:8<1484::AID-ELPS1484>3.0.CO;2-9

PMID:11386660

Abstract

A standard curve for the quantification of L-ascorbic acid (L-AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D-isoascorbic acid (D-IAA) as an internal standard. The minimum detection limits (MDLs) for L-AA and D-IAA were determined to be 1 and 2 microg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL-homocysteine. The recoveries for L-AA in these juices were between 95 and 105%.

摘要

建立了毛细管区带电泳（CZE）定量测定L-抗坏血酸（L-AA）的标准曲线，并以D-异抗坏血酸（D-IAA）为内标，对水果（柠檬、新奇士橙和菠萝）和菠菜中的抗坏血酸及总抗坏血酸进行了定量测定。在265 nm处，L-AA和D-IAA的最低检测限（MDLs）分别确定为1和2 μg/mL。在DL-同型半胱氨酸存在的情况下，对水果和菠菜中的脱氢抗坏血酸（DHAA）进行了定量。这些果汁中L-AA的回收率在95%至105%之间。