米曲霉IAM 2660菌株壳聚糖酶基因的克隆与特性分析
Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660.
作者信息
Zhang X Y, Dai A L, Kuroiwa K, Kodaira R, Nogawa M, Shimosaka M, Okazaki M
机构信息
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
出版信息
Biosci Biotechnol Biochem. 2001 Apr;65(4):977-81. doi: 10.1271/bbb.65.977.
A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.
从米曲霉IAM 2660中分离出编码壳聚糖酶（csnA）的基因的基因组拷贝。米曲霉csnA包含一个开放阅读框，该阅读框编码一个由245个氨基酸组成的多肽，计算分子量为26,500 Da。米曲霉csnA推导的氨基酸序列显示与其他真菌壳聚糖酶的氨基酸序列有广泛的相似性。
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