蛋白磷酸酶1和2A抑制剂对红系集落形成的影响：抑制剂特异性研究

Effect of inhibitors of protein phosphatase 1 and 2A on erythroid colony formation: an investigation of the specificities of inhibitors.

作者信息

Zhang W, Tamura J, Sakuraya M, Naruse T, Kubota K

机构信息

Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.

出版信息

J Int Med Res. 2001 Mar-Apr;29(2):114-8. doi: 10.1177/147323000102900208.

DOI:10.1177/147323000102900208

PMID:11393344

Abstract

To investigate the possible involvement of protein phosphatase (PP)1 and PP2A in the process of erythropoiesis, we assessed the effect of PP1 and PP2A inhibitors on erythroid colony formation using an in vitro colony formation assay. Okadaic acid (OKA), calyculin A (Cal-A) and tautomycin suppressed colony formation but 1-nor-okadaone did not. These results suggest that PP1 and PP2A both play an important role in erythropoiesis. Furthermore, higher concentrations of tautomycin were needed to suppress colony formation compared to concentrations of OKA and Cal-A. The target enzyme of inhibitors in erythropoiesis may be PP2A.

摘要

为了研究蛋白磷酸酶（PP）1和PP2A在红细胞生成过程中可能的作用，我们使用体外集落形成试验评估了PP1和PP2A抑制剂对红系集落形成的影响。冈田酸（OKA）、花萼海绵诱癌素A（Cal-A）和互隔交链孢酚单甲醚抑制了集落形成，但1-去甲冈田酸没有。这些结果表明PP1和PP2A在红细胞生成中均起重要作用。此外，与OKA和Cal-A的浓度相比，需要更高浓度的互隔交链孢酚单甲醚来抑制集落形成。红细胞生成中抑制剂的靶酶可能是PP2A。