Kunicki P K
Department of Clinical Biochemistry, National Institute of Cardiology, Warsaw, Poland.
J Chromatogr B Biomed Sci Appl. 2001 May 5;755(1-2):331-5. doi: 10.1016/s0378-4347(01)00053-6.
A HPLC-UV determination of loratadine in human plasma is presented. After simple liquid-liquid extraction with 2-methylbutane-hexane (2:1) and evaporation of organic phase the compounds were re-dissolved in 0.01 M HCl, evaporated again and finally separated on a Supelcosil LC-18-DB column. The analyses were done at ambient temperature under isocratic conditions using the mobile phase: CH3CN-water-0.5 M KH2PO4-H3PO4 (440:480:80:1, v/v). UV detection was performed at 200 nm with a limit of quantification of 0.5 ng/ml. The precision was found to be satisfactory over the whole range tested (0.5-50 ng/ml) with relative standard deviations of 2.3-6.3 and 5.2-14.1% for intra- and inter-assays, respectively.
本文介绍了一种采用高效液相色谱-紫外检测法测定人血浆中氯雷他定的方法。用2-甲基丁烷-己烷（2:1）进行简单液-液萃取，有机相蒸发后，将化合物重新溶解于0.01 M盐酸中，再次蒸发，最后在Supelcosil LC-18-DB柱上进行分离。分析在室温下等度条件下进行，流动相为：乙腈-水-0.5 M磷酸二氢钾-磷酸（440:480:80:1，v/v）。在200 nm处进行紫外检测，定量限为0.5 ng/ml。在所测试的整个范围内（0.5 - 50 ng/ml），精密度令人满意，批内和批间相对标准偏差分别为2.3 - 6.3%和5.2 - 14.1%。
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