李坏死环斑病毒重组外壳蛋白的制备
Preparation of recombinant coat protein of Prunus necrotic ringspot virus.
作者信息
Petrzik K, Mráz I, Kubelková D
机构信息
Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice.
出版信息
Acta Virol. 2001 Feb;45(1):61-3.
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
李坏死环斑病毒（PNRSV）的外壳蛋白（CP）基因被克隆到pET 16b载体中，并在大肠杆菌中表达。通过差速离心从全细胞裂解物中制备富含CP的组分。将在20,000×g下沉淀30分钟的组分用于制备兔抗CP血清。该抗血清的效价为1:2048，并在双抗体夹心ELISA（DAS-ELISA）中发生反应。
Zotero 插件