肝素结合蛋白HBp17对细胞DNA合成的双功能作用。

Bifunctional effects of heparin-binding protein HBp17 on DNA synthesis in cells.

作者信息

Chen J H, Wang X C, Sato J D

机构信息

Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, PR China.

出版信息

Cell Biol Int. 2001;25(6):567-70. doi: 10.1006/cbir.2000.0688.

DOI:10.1006/cbir.2000.0688

PMID:11407864

Abstract

A 17 kD heparin-binding protein (HBp17) has a biphasic dose-dependent effect on DNA synthesis in 3T3 cells. Maximal stimulation of DNA synthesis occurs at 8 ng/ml HBp17, but a half-maximal inhibition occurs at approximately 500 ng/ml. This inhibition can easily be reversed by addition of 400 pg/ml aFGF or 100 pg/ml bFGF, whereas EGF had no effect. This biphasic action of HBp17 was also seen in human umbilical vein endothelial cells (HUVEC), whereas it was not found in the malignant cell line, A431-AJC. The functional relationship between HBp17 and FGF is discussed.

摘要

一种17kD的肝素结合蛋白（HBp17）对3T3细胞的DNA合成具有双相剂量依赖性作用。DNA合成的最大刺激出现在8ng/ml的HBp17时，但在约500ng/ml时出现半数最大抑制。通过添加400pg/ml的aFGF或100pg/ml的bFGF可轻易逆转这种抑制，而EGF则无作用。HBp17的这种双相作用在人脐静脉内皮细胞（HUVEC）中也可见，而在恶性细胞系A431 - AJC中未发现。讨论了HBp17与FGF之间的功能关系。