Wang L, Higashiura K, Togashi N, Saitoh S, Ura N, Shimamoto K
Second Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
Hypertens Res. 2001 May;24(3):303-9. doi: 10.1291/hypres.24.303.
The aim of this study was to determine the effect of Jiang-Tang-Ke-Li (JTKL), a Chinese medicine used to treat diabetes mellitus, on insulin resistance and hypertension in fructose-fed rats (FFR). Six-week-old male Sprague-Dawley rats were fed either normal rat chow (control) or a fructose-rich chow (FFR) for 6 weeks. For the last 2 weeks of the 6-week period of either diet, the rats were treated by gavage with gum arabic solution as a vehicle (control or FFR) or JTKL (3.24 g/kg/day; FFR+JT), and then an euglycemic hyperinsulinemic glucose clamp technique was performed to estimate insulin sensitivity. Systolic blood pressure was measured each week of the 6-week period. At the end of the glucose clamp, the soleus and extensor digitorum longus (EDL) muscles were dissected out for determination of the role of tumor necrosis factor (TNF)-alpha by an ELISA assay. Systolic blood pressures in the FFR groups were significantly higher than that in the control group, although there was no effect on systolic blood pressure for the last 2 weeks of treatment with JTKL. The average rate of glucose infusion during the glucose clamp, as an index of insulin sensitivity (M value), was significantly lower in the FFR than in the control rats, and treatment with JTKL for 2 weeks significantly increased the M value to that of control. TNF-alpha levels were significantly higher in the soleus and EDL muscles of the FFR (480+/-46 and 570+/-45 pg/g wet tissue in the soleus and EDL muscles, respectively) than in those of the control rats (177+/-34 and 206+/-33 pg/g wet tissue in the soleus and EDL muscles, respectively; p<0.01). Treatment with JTKL for 2 weeks significantly lowered TNF-alpha levels to the control levels (189+/-22 and 239+/-92 pg/g wet tissue in the soleus and EDL muscles, respectively). The results suggest that the Chinese medicine JTKL improves insulin resistance and modulates TNF-alpha in the soleus and EDL muscles in hypertensive and insulin-resistant fructose-fed rats.
本研究旨在确定用于治疗糖尿病的中药降糖颗粒（JTKL）对喂食果糖的大鼠（FFR）胰岛素抵抗和高血压的影响。六周龄雄性斯普拉格-道利大鼠分别喂食普通大鼠饲料（对照组）或富含果糖的饲料（FFR）六周。在这两种饮食六周期间的最后两周，大鼠通过灌胃给予阿拉伯胶溶液作为赋形剂（对照组或FFR组）或JTKL（3.24克/千克/天；FFR+JT组），然后采用正常血糖高胰岛素葡萄糖钳夹技术评估胰岛素敏感性。在六周期间每周测量收缩压。在葡萄糖钳夹结束时，解剖比目鱼肌和趾长伸肌（EDL），通过酶联免疫吸附测定法（ELISA）确定肿瘤坏死因子（TNF）-α的作用。FFR组的收缩压显著高于对照组，尽管在JTKL治疗的最后两周对收缩压没有影响。作为胰岛素敏感性指标（M值），葡萄糖钳夹期间的平均葡萄糖输注速率在FFR组中显著低于对照组大鼠，而JTKL治疗两周可使M值显著升高至对照组水平。FFR组比目鱼肌和EDL肌中的TNF-α水平（比目鱼肌和EDL肌中分别为480±46和570±45皮克/克湿组织）显著高于对照组大鼠（比目鱼肌和EDL肌中分别为177±34和206±33皮克/克湿组织；p<0.01）。JTKL治疗两周可使TNF-α水平显著降低至对照组水平（比目鱼肌和EDL肌中分别为189±22和239±92皮克/克湿组织）。结果表明，中药JTKL可改善高血压和胰岛素抵抗的喂食果糖大鼠的胰岛素抵抗，并调节比目鱼肌和EDL肌中的TNF-α。
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