N-甲基对氨基苯甲酰谷氨酸-γ-谷氨酰-γ-酪氨酸（3-硝基）：一种内淬灭的荧光γ-谷氨酰水解酶底物。
N-Me-pAB-Glu-gamma-Glu-gamma-Tyr(3-NO(2)): an internally quenched fluorogenic gamma-glutamyl hydrolase substrate.
作者信息
Pankuch J J, Coward J K
机构信息
Departments of Chemistry and Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
出版信息
Bioorg Med Chem Lett. 2001 Jun 18;11(12):1561-4. doi: 10.1016/s0960-894x(01)00103-2.
A gamma-glutamyl tripeptide containing an internally quenched fluorophore has been synthesized and shown to be a substrate for recombinant rat gamma-glutamyl hydrolase. HPLC, LC-MS, and fluorescence spectra support the conclusion that selective hydrolysis occurs at the penultimate peptide bond. Preliminary data indicate that hydrolysis of this substrate can be monitored continuously to yield steady-state kinetic data.
一种含有内部猝灭荧光团的γ-谷氨酰三肽已被合成，并被证明是重组大鼠γ-谷氨酰水解酶的底物。高效液相色谱、液相色谱-质谱联用和荧光光谱支持这样的结论：选择性水解发生在倒数第二个肽键处。初步数据表明，该底物的水解可以连续监测以获得稳态动力学数据。
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