Trigueros S, Arsuaga J, Vazquez M E, Sumners D W, Roca J
Institut de Biologia Molecular de Barcelona, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Nucleic Acids Res. 2001 Jul 1;29(13):E67-7. doi: 10.1093/nar/29.13.e67.
We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate according to their compactness. The second gel dimension is run at high voltage, and DNA knots migrate according to other physical parameters such as shape and flexibility. In comparison with one-dimensional gel electrophoresis, this procedure segregates the knotted DNA molecules from other unknotted forms of DNA, and partially resolves populations of knots that have the same number of crossings. The two-dimensional display may allow quantitative and qualitative characterization of different types of DNA knots simply by gel velocity.
我们描述了一种二维琼脂糖凝胶电泳方法,该方法可提高打结DNA分子的分辨率。第一维凝胶在低电压下运行,DNA结根据其紧密程度迁移。第二维凝胶在高电压下运行,DNA结根据其他物理参数(如形状和柔韧性)迁移。与一维凝胶电泳相比,该方法将打结的DNA分子与其他未打结的DNA形式分离开来,并部分解析了具有相同交叉数的结群体。二维展示可能仅通过凝胶速度就允许对不同类型的DNA结进行定量和定性表征。