局部应用组织因子途径抑制剂可减少兔自体小动脉移植血管的内膜厚度。

Topically applied tissue factor pathway inhibitor reduced intimal thickness of small arterial autografts in rabbits.

作者信息

Sun L B, Utoh J, Moriyama S, Tagami H, Okamoto K, Kitamura N

机构信息

First Department of Surgery, Kumamoto University School of Medicine, Japan.

出版信息

J Vasc Surg. 2001 Jul;34(1):151-5. doi: 10.1067/mva.2001.113307.

DOI:10.1067/mva.2001.113307

PMID:11436089

Abstract

PURPOSE

The purpose of this study was to investigate whether topically applied tissue factor pathway inhibitor (TFPI) reduces intimal thickness and increases long-term patency of small arterial autografts in rabbits.

METHODS

An entire 10-mm long section of the left femoral artery was harvested and immersed in saline solution (control group, n = 10), 100 IU/mL of heparin (heparin group, n = 15), or 40 microg/mL of TFPI (TFPI group, n = 15) for 15 minutes. Then the graft was interposed to the right femoral artery. Patency rates were determined by flow measurements throughout the time course of the study, and the grafts were analyzed for measurement of intimal thickness at 3 months after operation. Immunohistochemical analysis was performed to examine whether topically applied TFPI binds to endothelial cells of the grafts.

RESULTS

Three-month postoperative patency rates were 10% in the control group, 47% in the heparin group, and 73% in the TFPI group. The TFPI group had a significantly higher patency rate than that of the control group (P <.005). Compared with the heparin group, the TFPI group had a significant reduction in intimal area (0.19 +/- 0.05 mm(2) vs 0.30 +/- 0.09 mm(2), P =.0051), in percentage of stenosis (35.7% +/- 7.7% vs 61.4% +/- 15.8%, P <.0001), and in intimal/media areas ratio (0.64 +/- 0.24 vs. 1.04 +/- 0.33, P =.0051). Immunohistologic analyses confirmed that topically applied TFPI bound to endothelial cells.

CONCLUSION

These results indicate that topically applied TFPI reduces intimal thickness and increases long-term patency of small arterial autografts in rabbits.

摘要

目的

本研究旨在调查局部应用组织因子途径抑制剂（TFPI）是否能减少家兔小动脉自体移植物的内膜厚度并提高其长期通畅率。

方法

切取左侧股动脉一整段10毫米长的血管，浸入盐溶液（对照组，n = 10）、100 IU/mL肝素（肝素组，n = 15）或40 μg/mL TFPI（TFPI组，n = 15）中15分钟。然后将移植物植入右侧股动脉。在整个研究过程中通过血流测量确定通畅率，并在术后3个月对移植物进行内膜厚度测量分析。进行免疫组织化学分析以检查局部应用的TFPI是否与移植物的内皮细胞结合。

结果

术后3个月，对照组的通畅率为10%，肝素组为47%，TFPI组为73%。TFPI组的通畅率显著高于对照组（P <.005）。与肝素组相比，TFPI组的内膜面积显著减小（0.19±0.05 mm² 对 0.30±0.09 mm²，P =.0051），狭窄百分比显著降低（35.7%±7.7% 对 61.4%±15.8%，P <.0001），内膜/中膜面积比显著降低（0.64±0.24 对 1.04±0.33，P =.0051）。免疫组织学分析证实局部应用的TFPI与内皮细胞结合。