印度对虾（Penaeus indicus H. Milne Edwards）血淋巴中一种天然凝集素的纯化与特性分析
Purification and characterization of a natural agglutinin in the hemolymph of the prawn Penaeus indicus H. Milne Edwards.
作者信息
Jayasree S
机构信息
Biochemistry and Genetic Engineering Research Unit, Cochin University of Science and Technology, Cochin, 682 022, India.
出版信息
J Invertebr Pathol. 2001 May;77(4):237-42. doi: 10.1006/jipa.2001.5027.
A natural agglutinin in the hemolymph of the marine prawn Penaeus indicus was isolated by gel filtration chromatography, purified using polyacrylamide gel electrophoresis, and characterized. Prawn agglutinin has a native molecular mass of 181 kDa and consists of two monomeric units (97 and 84 kDa), maintains some agglutinating activity over a wide pH range (7-9), and is inactivated at 85 degrees C. The agglutinin was denatured upon mixing with trichloroacetic acid, phenol, chloroform, and 45% ammonium sulfate. It was also sensitive to trypsin digestion. The results indicate that prawn agglutinin is proteinaceous in nature, with agglutinating, hemolytic, and antibacterial properties against marine bacteria and erythrocytes with carbohydrate binding sites.
通过凝胶过滤色谱法从印度对虾的血淋巴中分离出一种天然凝集素，经聚丙烯酰胺凝胶电泳纯化并进行表征。对虾凝集素的天然分子量为181 kDa，由两个单体单元（97 kDa和84 kDa）组成，在较宽的pH范围（7 - 9）内保持一定的凝集活性，在85℃时失活。该凝集素与三氯乙酸、苯酚、氯仿和45%硫酸铵混合后会变性。它对胰蛋白酶消化也敏感。结果表明，对虾凝集素本质上是蛋白质，对海洋细菌和红细胞具有凝集、溶血及抗菌特性，且具有碳水化合物结合位点。
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